WebTransferrin is a monomeric serum glycoprotein (∼80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe 3+ atoms via … Transferrin is a monomeric serum glycoprotein (∼80,000 daltons) that … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebAug 13, 2024 · Transferrin uptake assay. Mouse peritoneal macrophages were co-cultured with Hepa1-6/shNT or Hepa1-6/shTfrc cells in a 0.4 μm Transwell system. Cells were starved in serum-free DMEM for 24 h. After being washed with PBS three times, cells were incubated in serum-free DMEM containing 5 μM FITC-labeled holo-transferrin for 2 h.
FITC Anti-Transferrin Receptor antibody [YTA 74.4] …
Web125I- and 31lI-labeled transferrin (1251- and 3lI-transferrin) were <17%, respectively, of the peak intensities at 490 nm. both -600-900 cpm/ng. Gradients. After incubation with the appropriate ligand at Labeling of .Transferrin with Fluorescein ;Isothiocyanate either 0?C or 37?C, cells (2.5 x 107 cells/ml) were washed thor-(FITC). WebFITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. Fluorescein … chk bouw
LWJ-M30, a conjugate of DM1 and B6, for the targeted therapy of ...
WebFeb 25, 2016 · The aim of this work was to assess the role of transferrin (Tf) modification and phosphatidylcholine (PC) composition on the properties of liposomes containing hydroxypropyl-β-cyclodextrin (HP-β-CD). Fluorescence dye, FITC, was conjugated with HP-β-CD to facilitate the analysis. WebWe used a fluorescence conjugated transferrin (FITC-Tf) to monitor the trafficking of TfR1. Cells were incubated with 20 μg/ml FITC-Tf on ice for 20 min. At low temperatures, Tf still bound to TfR1 on the cell surface, but endocytosis was terminated. After the unbound FITC-Tf was washed away, the cells were transferred to 37 C to reinitiate ... WebFluorescein (FITC) Fluorescence SpectraViewer. Fluorescein derivatives are the most common fluorescent reagents for biological research because of their high absorptivity, … grassley\\u0027s age