Click-it edu imaging kits
WebThis EdU assay kit can also be multiplexed with antibodies against surface and intracellular markers. However, some of these reagents or antibodies may not be compatible with the Click-iT™ EdU detection reagent and may need some additional steps to make the reagent or antibody compatible with the Click-iT™ EdU detection reagent (see Table 2 for WebMar 9, 2015 · The EdU assay kit can also be multiplexed with surface and intracellular marker detection using antibodies (see Table 2 for details). Finally, unlike the BrdU assay, which relies upon antibodies which can exhibit nonspecific binding, the Click-iT® EdU …
Click-it edu imaging kits
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WebThe Click-iT EdU Alexa Fluor 488 Imaging Kit is a superior alternative to traditional proliferation assays that is optimized for fluorescence microscopy applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized … WebMar 31, 2015 · After fixation, cells were stained with EdU according to the protocol for Click-iT EdU Imaging Kits (Life Technologies). Following EdU staining, cells were stained with RPA and CENPF as described above. Microscopic images were taken by an Applied Precision DeltaVision OMX microscope using the settings for conventional image capture …
WebInvitrogen™ Click-iT™ Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 594 dye. Designed to label and detect incorporated EdU and perform cell cycle analysis on samples from adherent cells. Includes: EdU (5-ethynyl-2' -deoxyuridine), Alexa Fluor dye-picolyl … WebMeasuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal resu...
WebFeb 21, 2015 · I'm using the Click-it Edu Imaging kit from Life Technologies to look at proliferation in the olfactory epithelium of E15 mouse embryos. When I initially tested the conditions, I had decent ... WebPrepare stock solutions. Allow vials to warm to room temperature. Add 2 mL DMSO (Component C) or an aqueous solution to Component A to make a 10 mM EdU stock solution. Store at –20°C. Make 1X Click-iT EdU reaction buffer by transferring the …
WebClickTech EdU Cell Proliferation Kit for Imaging 7 7. EdU detection In this protocol, 500 µL of the reaction cocktail per coverslip are used. Also smaller volumes can be used, as long as the reaction components are applied in the same ratios. 7.1. Prepare the reaction cocktail in the same order as described in the following table. If the
http://www.biodee.net/Show/index/cid/63/id/21736.html ppn vs tpn osmolarityWebMay 17, 2024 · EdU labeling. For EdU labeling, mice were injected with 25 µg of EdU per gram of body weight and euthanized after 1 h. Tongues were fixed overnight in 4% PFA and embedded in paraffin. After de-waxing, paraffin sections were incubated with the Click-iT EdU Imaging Kit (Invitrogen, CA, USA). ppnlt japanWebNov 25, 2024 · We use the Click-iT ® EdU Imaging Kits from Invitrogen. EdU can also be obtained from Sigma-Aldrich. EdU is prepared as a 10 mM stock solution in DMSO and stored at −20 °C. The stock solution is stable for 1 year . ... Click-iT ® EdU Imaging Kits from Invitrogen. See the Methods section for the preparation of the reaction cocktail. bannerman rd tallahasseeWebClick-iT® Plus EdU Imaging Kits 6 Detect EdU Note: This protocol uses 500 µL of Click-iT® Plus reaction cocktail per coverslip. A smaller volume can be used as long as the remaining reaction components are maintained at the same ratios. ®4.1 Prepare 1X Click-iT EdU buffer additive (see Table 3, below) by diluting the 10X solution (prepared in step … ppnpn kemenkeuWebwahoo.nsm.umass.edu bannerman shotgunWebDec 15, 2014 · Click-iT EdU Imaging Kits EdUdetection Note: protocoluses 500 Click-iTreaction cocktail per coverslip. smallervolume can remainingreaction components sameratios. 4.1Prepare 1X Click-iT EdU buffer additive (see Table 10Xsolution (prepared step1.5) 1:10 deionizedwater. Prepare solutionfreshand use sameday. 4.2Prepare Click … bannerman truck rampWebFor convenient processing, use 6-well plates. After metabolic labeling, remove the media and add 1 mL of 3.7% formaldehyde in PBS to each well containing the coverslips. Incubate for 15 minutes at room temperature. Remove the fixative and wash the cells in each well twice with 1 mL of 3% BSA in PBS. bannerman\u0027s catalog